Transportation Security Administration(TSA) has been working for years with the ROA's administration to provide a safe environment and flight for passengers. From 2018, TSA implemented stricter screening procedures for carry-on bags and items at Roanoke-Blacksburg Regional Airport.

At security counters, travelers are asked to remove any personal belongings, electronic devices that are larger than a cell phone. Visitors have to put these items in the containers for X-ray screening. TSA officers ask passengers to remove these kinds of things from their carry-on bags too. They have to put their personal belongings in the bins with nothing on top of them. This way, it will be easier for the X-ray scanners to check the container. TSA employees guide travelers through this process and recommend to organize carry-on bags before coming at checkpoints.

ROA recommends passengers arrive at the airport 90 minutes before departure time. For passengers registered on TSA Precheck, there is a separate line. To check a traveler's identity, TSA officers accept government-issued I.D., driver's license, U.S. passport, U.S. passport card, permanent resident card, and so on.